Research Article of International Journal of Food and Nutrition Research
Effects of Artepelin-C Supplementation Present in Propolis Related to Inflammatory Processes in Physically Active Individuals
Erico Chagas Caperuto1, Maria Aparecida Nicoletti2, Michael Martini Silva1, Tony Cezar Goularte1, Jeferson Oliveira Santana1, Natalia Gusmão1, Catarina Andrade Barbosa3, Helenice de Souza Spinosa4, Juliana Weckx Peña Muñoz1, André Rinaldi Fukushima4,5
1Universidade São Judas Tadeu-R. Taquari, São Paulo/SP, Brazil; 2Departamento de Farmácia. Faculdade de Ciências Farmacêuticas. Universidade de São Paulo, São Paulo/SP, Brazil; 3Departamento de estudo de atividade física adaptada – Faculdade de Educação Física, Universidade de Campinas, Campinas/SP, Brasil; 4Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo/SP, Brazil;5Faculdade São Bento-Largo de São Bento, s/n – Centro, São Paulo/SP, Brazil
The scientific literature shows that propolis has both anti-inflammatory and antibacterial activities and is widely used in phytotherapic therapy. In this context, its main objective is to evaluate the inflammation recovery process in physically active individuals, from two groups, with or without propolis intake. Volunteers had their food, blood and pain parameters evaluated with or without propolis intake. The trial used seven male volunteers undergoing specific training in two 30-day protocols, one using placebo and another using propolis which contains Artepelin-C (chemically 3,5-diprenyl-p-coumarin acid, which is one of the main phenolic acids present in the green propolis extract). Participants between 18 and 35 years old under no medication should have had at least a 6-month workout. Performance physical tests were applied, body composition measurements and blood collection were taken and a 24-hour food recall and food frequency questionnaire were carried out at São Judas Tadeu University. All volunteers were asked to register their food intake during the 30-day protocol and data were analyzed by using ANOVA and Students T-test for paired samples at <0.05 significance level set. A significant lymphocytes increase during placebo protocol and a significant reduction of these cells during propolis protocol were observed. Concerning propolis and placebo protocols, volunteers showed less pain during the former, which means that propolis is highly beneficial for those practicing intense physical exertion. Artepelin-C present in propolis protocol and having a modulator effect in inflammatory processes enables a higher intense physical exertion more easily, due to its property to minimize pain.
Keywords: Physical activity; Inflammation process; Body composition; Propolis; Artepelin-C; Blood tests; Pain perception and evaluation.
How to cite this article:
Erico Chagas Caperuto, Maria Aparecida Nicoletti, Michael Martini Silva, Tony Cezar Goularte, Jeferson Oliveira Santana, Natalia Gusmão, Catarina Andrade Barbosa, Helenice de Souza Spinosa, Juliana Weckx Peña Muñoz, André Rinaldi Fukushima.Effects of Artepelin-C Supplementation Present in Propolis Related to Inflammatory Processes in Physically Active Individuals. International Journal of Food and Nutrition Research, 2019; 3:22. DOI: 10.28933/ijnfr-2019-01-1806
 Souglis A, Bogdanis GC, Giannopoulou I, Papadopoulos Ch, Apostolidis N, 2015. Comparison of inflammatory responses and muscle damage indices following a soccer, basketball, volleyball and handball game at an elite competitive level. Res Sports Med.;23(1):59-72. DOI: 10.1080/15438627.2014.975814
 Anglem N, Lucas SJ, Rose EA, Cotter JD, 2008. Mood, illness and injury responses and recovery with adventure racing. Wilderness Environ Med., Spring;19(1):30-8. DOI: 10.1580/07-WEME-OR-091.1
 Barbas I, Fatouros IG, Douroudos II, Chatzinikolaou A, Michailidis Y, Draganidis D, Jamurtas AZ, Nikolaidis MG, Parotsidis C, Theodorou AA, Katrabasas I, Margonis K, Papassotiriou I, Taxildaris K, 2011. Physiological and performance adaptations of elite Greco-Roman wrestlers during a one-day tournament. Eur J Appl Physiol., Jul;111(7):1421-36. DOI: 10.1007/s00421-010-1761-7.
 Neefkes-Zonneveld CR, Bakkum AJ, Bishop NC, van Tulder MW, Janssen TW, 2015. Effect of long-term physical activity and acute exercise on markers of systemic inflammation in persons with chronic spinal cord injury: a systematic review. Arch Phys Med Rehabil. Jan;96(1):30-42. DOI: 10.1016/j.apmr.2014.07.006.
 Corrêa FR, Schanuel FS, Moura-Nunes N, Monte-Alto-Costa A, Daleprane JB, 2017. Brazilian red propolis improves cutaneous wound healing suppressing inflammation-associated transcription factor NFκB. Biomed Pharmacother., Feb;86:162-171. DOI: 10.1016/j.biopha.2016.12.018
 Rufatto LC, dos Santos DA, Marinho F, Henriques JAP, Ely MR, Moura S, 2017. Red propolis: Chemical composition and pharmacological activity. Asian Pac J Trop Biomed., 7(7): 591–598. Available from: http://dx.doi.org/10.1016/j.apjtb.2017.06.009
 Silva MR, Oliveira RGS, Vasconcelos MA, 2018. Avaliação do efeito anticarcinogênico da própolis verde (Artepelin C) por meio do teste warts em células somáticas de Drosophila melanogaster. Revista Perquirere, jan./abr;15(1):285-296. Available from: http://perquirere.unipam.edu.br/documents/1833550/2242494/Avalia%C3%A7%C3%A3o+do+efeito+anticarcinog%C3%AAnico+da+pr%C3%B3polis+verde.pdf/795f27e2-f77f-497e-b8be-54918f45d6d5
 Perini TA, de Oliveira GL, Ornellas JS, de Oliveira FP, 2005. Cálculo do erro técnico de medição em antropometria. Rev Bras Med Esporte, 11(1):81-5. Available from: http://www.scielo.br/pdf/rbme/v11n1/24109.pdf
 Lohman TG, Roche AF, Martorell R, 1988. Anthropometric standardization reference manual. Champaign: Human Kinetics.
 Kraemer WJ, Gordon SE, Fleck SJ, Marchitelli LJ, Mello R, Dziados JE, Friedl K, Harman E, Maresh C, Fry AC, 1991. Endogenous anabolic hormonal and growth factor responses to heavy resistance exercise in males and females. Int J Sports Med., Apr;12(2):228-35. DOI: 10.1055/s-2007-1024673
 Gibala MJ, McGee SL, 2008. Metabolic adaptations to short-term high-intensity interval training: a little pain for a lot of gain? Exerc Sport Sci Rev., Apr;36(2):58-63. DOI: 10.1097/JES.0b013e318168ec1f.
 Fleck SJ, Kraemer WJ, 2006. Fundamentos do treinamento de força muscular. 3ª ed. Porto Alegre: Artmed.
 Fisberg RM, Martini LA, Slater B, 2005. Métodos de inquéritos alimentares. In: Fisberg RM, Slater B, Marchioni DML, Martini LA. Inquéritos alimentares: métodos e bases científicos. São Paulo: Manole.
 Buzzard DM. 24-hour dietary recall and food record methods. In: Willett W. Nutritional epidemiology. 2.ed. New York: Oxford University Press, 1998. p.51-67.
 Martines JE, Grassi DC, Marques LG, 2011. Analysis of the applicability of different pain questionnaires in three hospital settings: outpatient clinic, ward and emergency unit. Rev Bras Reumatol.,51(4):299-308. Available from: http://dx.doi.org/10.1590/S0482-50042011000400002
 Tiggemann CL, Pinto RS, Kruel LFM, 2010. A percepção de esforço no treinamento de força. Rev Bras Med Esporte [online]; 16(4):301-309. ISSN 1517-8692. Available from: http://dx.doi.org/10.1590/S1517-86922010000400014
 Borg G, 2000. Escalas de Borg para a Dor e Esforço Percebido. 1.ed. São Paulo: Manole.
 IBM. Statistical package for the Social Sciences (SPSS). Version 20.0
 de França HS, Branco PA, Guedes Junior DP, Gentil P, Steele J, Teixeira CV. The effects of adding single-joint exercises to a multi-joint exercise resistance training program on upper body muscle strength and size in trained men. Appl Physiol Nutr Metab. 2015 Aug;40(8):822-6. DOI: 10.1139/apnm-2015-0109.
 Guyton AC, Hall JE. Tratado de fisiologia médica. 13.ed. 2017, Rio de Janeiro, Elsevier.
 Crome SQ, Ohashi PS, 2018. Immunoregulatory functions of innate lymphoid cells. Journal for ImmunoTherapy of Cancer. 19 Nov; 6(121):1-4. DOI: 10.1186/s40425-018-0433-8.
 Kratofil RM, Kubes P, Deniset J F, 2017. Monocyte conversion during inflammation and injury. Arteriosclerosis, Thrombosis, and Vascular Biology, Calgary, 37(1):35-42. DOI: 10.1161/ATVBAHA.116.308198.
 Playfair JHL, Chain BM, 2013. Imunologia Básica: Guia ilustrado de conceitos fundamentais. 9. ed. Barueri: Manole.
 Prussin C, Metcalfe DD, 2003. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol. Feb;111(2 Suppl):S486-94. Available from: https://doi.org/10.1067/mai.2003.120
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