Vânia Mattoso1*; Gabrielle Rocha1, Sérgio Barroso1, Vilma Blondet de Azeredoa Adenilson de Souza da Fonseca2,3

1Federal Fluminense University, Nutrition College, Rio de Janeiro, Brazil; 2University of the State of Rio de Janeiro, Department of Biophysics and Biometrics, Roberto Alcântara Gomes Biology Institute, Brazil; 3Department of Physiological Sciences, Biomedical Institute, Federal University of the State of Rio de Janeiro, Frei Caneca Street, 94, Rio de Janeiro, 20211040, Brazil.

International Journal of Food and Nutrition Research

Introduction: In addition to its anti-cancer action, p53 and ATM play an important role in oxidative balance control, promoting cell repair and survival. High fat diets can lead to increased production of reactive oxygen species (EROS). Grape polyphenols seem to reduce EROS and restore oxidative balance, favoring the performance of p53 and ATM. Objetive: The aim of this study was to investigate the antioxidant properties of high polyphenols beverages associated with a high fat diet in mRNA levels of p53 and ATM. Methods: Fifty female rats were divided into five groups: Control Group (CG) – control diet (4% fat); High fat diet group (HFD) – high fat diet (20% fat); Grape Juice Group (GJ) – grape juice (15 ml/day) + high fat diet; Red Wine Group (RW) – red wine (10 ml/day) + high fat diet; Resveratrol Solution Group (RS) – resveratrol solution (15 ml/day) + high fat diet. Eight weeks later, muscular and adipose tissue were collected and subjected to PCR analysis. Results: In muscular tissue, the highest p53 mRNA expression was found in the GJ and VT group, and not in the SR as expected. In adipose tissue, GJ presented the highest expression among all groups. TpATM expression was higher in the HFD, both in adipose and muscle tissue. Treatment with high polyphenols beverages normalized TpATM expression, especially in adipose tissue. Conclusion: In this experimental model, high fat diet alters ATM mRNA levels, but does not change p53 mRNA levels. Grape juice and red wine showed to be the most effective to increase TP53 mRNA levels, possibly due to a set of bioactive compounds that acts synergistically. Additionally, rich polyphenols beverages normalizes ATM mRNA levels, mainly in adipose tissue.

Keywords: p53, ATM, high fat diet, grape polyphenols

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Vânia Mattoso; Gabrielle Rocha, Sérgio Barroso, Vilma Blondet de Azeredoa Adenilson de Souza da Fonseca. EFFECT OF HIGH POLYPHENOLS BEVERAGES ON mRNA LEVELS FROM TP53 AND ATM GENES.International Journal of Food and Nutrition Research, 2019; 3:23.


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