International Journal of Bioscience and Medicine

  • Environmental Enrichment in the ISS Rodent Habitat Hardware System

    Responses of animals exposed to microgravity during in-space experiments were observed via available video recording stored in the NASA Ames Life Sciences Data Archive. These documented observations of animal behavior, as well as the range and level of activities during spaceflight, clearly demonstrate that weightlessness conditions and the extreme novelty of the surroundings exert damaging psychological stresses on the inhabitants. In response to a recognized need for in-flight animals to improve their wellbeing we propose to reduce such stresses by shaping and interrelating structures and surroundings to satisfying vital physiological needs of inhabitants. A Rodent Habitat Hardware System (RHHS) based housing facility incorporating a tubing network system, to maintain and monitor rodent health environment with advanced accessories has been proposed. Placing mice in a tubing-configured environment creates more natural space-restricted nesting environment for rodents, thereby facilitating a more comfortable transition to living in microgravity. A sectional tubing structure of the RHHS environment will be more beneficial under microgravity conditions than the provision of a larger space area that is currently utilized. The new tubing configuration was found suitable for further incorporation of innovative monitoring technology and accessories in the animal holding habitat unit which allow to monitor in real-time monitoring of valuable health related biological parameters under weightlessness environment of spaceflight.

  • Prevalence and Drug Resistance Patterns of Staphylococcus aureus in Food Producing Animals, Their Products and Humans

    Staphylococcus aureus is a gram positive bacterium that belongs to coagulase positive staphylococcus. It is widely distributed in the environment and causes diseases due to direct infection or due to the production of toxins by the bacteria. The prevalence of S. aureus ranges from 4% to 83% in different countries of the world from samples of food producing animals and their products. In Ethiopia the lowest and highest prevalence is reported as 4.2% and 48.75%, respectively. All mammals and birds are susceptible to colonization with S. aureus. Staphylococcus aureus can be treated with a wide range of antibiotics but there are efficient and inefficient antibiotics. Antibiotic resistance tests that have been conducted in different countries revealed that S. aureus were highly resistant to Ampicillin, Cloxacillin and Penicillin and less susceptible to Vancomycin and Rifampicin. The emergence of methicillin resistant Staphylococcus aureus in animal and human has become a worldwide problem.

  • Thaumatococcus daniellii Benn. (Marantaceae) Leaf Methanol Extract Possessed Hepatoprotective Effect Against Acetaminophen-induced Liver Injury in Albino Rats

    [Background]Acetaminophen or Paracetamol is widely used as analgesic and antipyretic or antimalarial drug, but at high dose it leads to undesirable side effects, such as hepatotoxicity. [Objective]This present study demonstrates the in vivo hepatoprotective effect of Thaumatococcus daniellii against acetaminophen induced liver toxicity. [Methods] Acetaminophen- induced liver injury was evaluated by an increase in serum AST (or SGOT), ALT (or SGPT), ALP activity and bilirubin level accompanied by significant decrease in albumin level. [Results] Acetaminophen hepatoxicity was manifested by an increase in lipid peroxidation, depletion of reduced glutathione (GSH) and catalase activity in liver tissue. Oral administration of the plant extract protects the rats against acetaminophen induced liver injury by increased lipid peroxidation, restored altered serum marker enzymes and antioxidant level to normal liver morphology.[Conclusion]The results showed that T. daniellii leaf extract displayed significant liver healing efficacy against acetaminophen induced hepatotoxicity in rats. The result further affirm the use of the plant as an ethno-medicinal prescription for liver related injury.

  • Soluble fibrin as a biomarker for intrahepatic microthrombosis in acute-on-chronic liver failure

    Background: There is a lack in finding the precipitating factor in acute –on chronic liver failure (ACLF)insult in large number of patients and either this factor is hepatic or extra hepatic origin. Aim of the work : Our study aiming to evaluate the potential usefulness of a new plasma soluble fibirin polymer (SF) assay for diagnosing the possibility of occurrence of intrahepatic microthrombosis as a cause of ACLF in patients with chronic hepatitis C virus. Patients & Methods: The study was carried out in Zagazig University Hospital, internal medicine department in collaboration with microbiology department from February 2015 tell November 2015. 50 patients having chronic hepatitis c virus was enrolled in this study with ACLF developing new onset ascitis in 15 patient encephalopathy in 12 patients, jaundice in12 and elevated INR in 11 patients all having regular follow-up in hepatology clinic in Zagazig university Hospital with stable clinical course in the previous three months. Control subject was classified as normal subject 20 and 30 patients with compensated chronic hepatitis C virus infection. All patients and control groups were subjected to full history, complete clinical examination and laboratory tests including CBC, INR, serum albumin, serum bilirubin, liver enzymes, ascitic fluid examination and culture, blood culture, alpha-fetoprotein, d D-dimer, thrombin generation and soluble fibrin polymer., abdominal ultrasound, Doppler ultrasound for portal vein. Results: Our results showed significant difference between patients group and both control groups regarding SF and D-dimer, also there were significant differences in patients group and other groups regarding ALT, total bilirubin especially direct bilirubin. There were marked reduction in portal flow mean velocity in patients group and other groups and we recorded further reduction in the portal flow mean velocity in patients group after 2 weeks from the starting time. There is significant positive correlation between SF and D-dimer…

  • Optimization of fermentation conditions for the production of 2,3-butanediol by Klebsiella pneumonia ZH-1 using response surface methodology

    [Background] It has been studied that the yield of 2,3-butanediol (2,3-BD) producing strains is low and does not meet the requirements of industrial production of 2,3-BD. [Objective] It was important to improve the production of 2,3-BD by Klebsiella pneumonia ZH-1 in shaking flask. [Methods] The effects of temperature, pH and rotating speed on the production of 2,3-BD were studied by single factor test and response surface method. [Results] The optimal cultivation conditions stimulating the maximal production of 2,3-BD were as follow: initial pH, 7, temperature, 37 oC and rotating speed, 140 r/min. Under this optimized conditions, the predicted maximal 2,3-BD yield was 21.54 g/L, whereas the yield of 2,3-BD can reach to 22.04 g/L after the application of response surface methodology. [Conclusion] Response surface methodology was a promising method for optimization of 2,3-BD production.

  • Prevalence and Associated Factors of Antiepileptic Drugnonadherence Among Epileptic Patients Attending at Out Patient Departement of Dilla University Referral Hospital, Dilla,gedeo,snnpr, Southern Ethiopia

    Background: Antiepileptic drugs are effective in the treatment of epilepsy, but poor adherence to medication is major problem to sustained remission and to functional restoration. Poor adherence to treatment is one of many reasons for pharmacological treatment failure and seizure recurrence. Even though there were studies on the magnitude and associated factors of Antiepileptic drugs non-adherence, there is a shortage of published information regarding the prevalence and associated factors of Antiepileptic drugs none-adherence in Ethiopia. Objective: To assess prevalence and associated factors of antiepileptic drug none-adherence among epileptic patients attending at Dilla University Referral Hospital Methods: Institutional based cross sectional study design was conducted at Dilla University Referral Hospital from March to May, 2016. A total of 265 individuals was selected by simplerandom sampling method and interviewed by using structured questionnaire. 8 itemMorisky Medication Adherence Scalewas used to assess the prevalence of antiepileptic drug non adherence. Data was coded and exported to SPSS version 20 for analysis. Results: The prevalence of Antiepileptic’s drug none-adherence in this study was 38.1% and getting medication by payment [AOR2.009, 95%,CI:1.044, 3.868], Patients who did not got health information about(their illness, duration of treatment and drug side effect) [AOR=0.319,95%,CI:0.184,0.534], poor social support [AOR=3.06, 95%, CI: 1.47-6.37], skip dose [AOR=2.462,95%,CI:1.375,4.407], patients who were on treatment for 2-5 years [AOR=1.48, 95%, CI: 0.722,3.035] were found to be significantly associated (p. Conclusion: The prevalence of antiepileptic drug none-adherence among patients with epilepsy disorder was found 38.1%. Getting medication by payment, did not receive health information about (the illness, duration of treatment, medication side effect), skip dose, on treatment for 2-5 years, and poor social support were found to be the independent predictor of antiepileptic drug none-adherence.