In-vitro Antioxidant Capacities and Genetic Classification of Indonesian Selected Pigmented Rice

In-vitro Antioxidant Capacities and Genetic Classification of Indonesian Selected Pigmented Rice

Erlin Susilowati1, Addieni Zulfa Karimah1, Sandy Pradipta2, Bella Rhea Lavifa Sanjaya2, Mohammad Ubaidillah1,2,3, Tri Agus Siswoyo1,2,3*

1Graduate School of Biotechnology, University of Jember, Jember-68121, Indonesia. 2The Center of Excellence on Crop Industrial Biotechnology (PUI-PT BioTIn), University of Jember, Jember-68121, Indonesia. 3Faculty of Agriculture, University of Jember, Jember 68121, Indonesia

Rice is a world-famous cereal food divided into pigmented and non-pigmented rice. Pigmented rice is popular as healthier food than non-pigmented rice due to its potency as an antioxidant. Nevertheless, the potential of pigmented rice has not been widely studied. Indonesian selected pigmented rice protein’s antioxidant potential and the non-protein compound were in-vitro studied. The antioxidant potencies were evaluated by extracting fresh seeds of nine pigmented rice (Aek Sibundong, Beureum Taleus, Gogo Niti-2, Lamongan-1, Merah SP, Merah Wangi, Mota, Ketan Hitam-2, and Super Manggis) and non-pigmented rice (IR-64) as control. Various free radical scavenging methods to determine the antioxidant activity (ABTS•+, DPPH•, OH• and O2-) were conducted. Meanwhile, the genetic classification was performed by a simple sequence repeat (SSR) marker to determine the relationship between varieties. The results showed that protein of Ketan Hitam-2 had the highest ABTS•+ radical scavenging (98.06%), followed by Beureum Taleus (42.54%). Ketan Hitam-2 protein also showed the highest OH• and O2- activities (43.49% and 6.02%, respectively). The highest DPPH• potency of the non-protein compounds also shown by Ketan Hitam-2 (32.23%) with the activity of OH• and O2- (20.63% and 14.56%, respectively). These results showed that Ketan Hitam-2 has the highest potency as an antioxidant, which could be recommended as a nutraceuticals compound.

Keywords: Antioxidant; Pigmented rice; SSR marker

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Erlin Susilowati, Addieni Zulfa Karimah, Sandy Pradipta, Bella Rhea Lavifa Sanjaya, Mohammad Ubaidillah, Tri Agus Siswoyo. In-vitro Antioxidant Capacities and Genetic Classification of Indonesian Selected Pigmented Rice. American Journal of Agricultural Research, 2021; 6:107. DOI: 10.28933/ajar-2020-11-2505


1. Sutee Chutipaijit, Suriyan Cha-Um, Kanokporn Sompornpailin. Differential accumulations of proline and flavonoids in indica rice varieties against salinity. Pak. J. Bot, 2009; 41(5): 2497-2506.
2. Gui Fang Deng, Xiang Rong Xu, Yuan Zhang, Dan Li, Ren You Gan, Hua Bin Li. Phenolic compounds and bioactivities of pigmented rice. Crit rev food sci nutr, 2013; 53(3): 296–306.
3. Pinderpal Kaur, Sukhvinder Singh Purewal, Kawaljit Singh Sandhu, Maninder Kaur. DNA damage protection : an excellent application of bioactive compounds. Bioresources and Bio- process, 2019; 6(2):1-11.
4. Ryan J. Elias, Sarah S. Kellerby, Eric A. Decker. Antioxidant activity of proteins and peptides antioxidant activity of proteins and peptides. Critical Reviews in Food Science and Nutrition, 2008; 48:430–441.
5. Corneliu Tanase, Sanda Cosarcă, Daniela-Lucia Muntean. A critical review of phenolic com- pounds extracted from the bark of woody vascular plants and their potential biological activity. Molecules, 2019; 24 (6):1-18.
6. Cristina Romera-Castillo, Rudolf Jaff´e. Free radical scavenging (antioxidant activity) of na- tural dissolved organic matter. Marine Chemistry, 2015; 177:668–676.
7. Nur Siti Kurniasih, Ratna Susandarini, Febri Adi Susanto, Tri Rini Nuringtyas, Glyn Jenkins, Yekti Asih Purwestri. Characterization of indonesian pigmented rice (Oryza sativa) based on mor- phology and single nucleotide polymor- phisms. Biodiversitas, 2019; 20 (4): 1208–1214.
8. Maria Lucia Carneiro Vieira, Luciane Santini, Augusto Lima Diniz, Carla de Freitas Munhoz. Microsatellite markers: What they mean and why they are so useful. Genetics and Molecular Biology, 2016; 39(3): 312–328.
9. Nuria Pedrol Bonjoch, PR Tamayo. Protein content quantification by bradford method. In: handbook of plant ecophysiology techniques. Springer, Dordrecht, 2001; pp. 283–295.
10. Suman Chandra, Shabana Khan, Bharathi Avula, Hemant Lata, Min Hye Yang, Mahmoud A. Elsohly, Ikhlas A. Khan. Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: A comparative study. Evidence-based Complement Altern Med, 2014; 2014: 1-9.
11. K Lalhminghlui, Ganesh Chandra Jagetia. Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth in vitro. Futur Sci OA, 2018; 4(2): 1-12.
12. Zeynep Akar, Murat Küçük, Hacer Doğan, H. A new colorimetric DPPH• scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs. J Enzyme Inhib Med Chem, 2017; 32 (1): 640-647.
13. Kalaisezhiyen Pavithra, Sasikumar Vadivukkara- si. Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Science and Human Wellness, 2015; 4 (1): 42-46.
14. Xueyan Tang, Zhiyong He, Yanfeng Dai, Youling L Xiong, Mingyong Xie, Jie Chen. Peptide fractionation and free radical scavenging activity of zein hydrolysate. J Agric Food Chem, 2010; 58 (1): 587–593.
15. Xin Xu, Shinji Kawasaki, Tatsuhito Fujimura, Chuntai Wang. A protocol for high-throughput extraction of DNA from rice leaves. Plant Molecular Biology Reporter, 2005; 23 (3): 291-295.
16. Lilit Garibyan, Nidhi Avashia. 2013; Polymerase chain reaction. J Invest Dermatol, 2013, 133(3):1-4.
17. Yodmanee, S., Karrila, T., and Pakdeechanuan, P. Physical, chemical and antioxidant properties of pigmented rice grown in Southern Thailand. International Food Research Journal, 2011; 18 (3): 901–906.
18. Sarah S. Kellerby, D.Julian Mcclements, Eric A. Decker. Role of proteins in oil-in-water emulsions on the stability of lipid hydroperoxides. Journal of Agricultural and Food Chemistry, 2006; 54: 7879–7884.
19. Senji Sakanaka, Yumi Tachibana. Food chemi- stry active oxygen scavenging activity of egg-yolk protein hydrolysates and their effects on lipid oxidation in beef and tuna homogenates. Food Chemistry, 2006; 95: 243–249.
20. Ute Panzenbock, Roland Stocker. Formation of methionine sulfoxide-containing specific forms of oxidized high-density lipoproteins. Biochimica et Biophysica Acta, 2005; 1703: 171–181.
21. Sadaf Yaqoob, Bushra Sultana, Muhammad Mushtaq. In vitro antioxidant activities of Trian- thema portulacastrum L. hydrolysates. Preven- tive Nutrition and Food Science, 2014; 19(1): 27–33.
22. Song-Hee Nam, Sun Phil Choi, M. Y. Kang, Hee-Jong Koh, Nobuyuki Kozukue, Mendel Friedman. Antioxidative activities of bran extracts from twenty one pigmented rice cultivars. Food Chemistry, 2006; 94 (4): 613–620.
23. Barry Halliwell, Matthew Whiteman. Measuring reactive species and oxidative damage in vivo and in cell culture: How should you do it and what do the results mean?. British Journal of Pharmacology, 2004; 142(2): 231–55.
24. Jakub Treml, Karel ˇSmejkal. Flavonoids as potent scavengers of hydroxyl radicals. Com- prehensive Reviews in Food Science and Food Safety, 2016; 15 (4): 720–738.
25. Supaluck Kraithong, Suyong Lee, Saroat Rawdkuen. Physicochemical and functional properties of Thai organic rice flour. Journal of Cereal Science, 2018; 79: 259–66.
26. D. K. Patel, R. Kumar, D. Laloo, S. Hemalatha. Natural medicines from plant source used for therapy of diabetes mellitus: An overview of its pharmacological aspects. Asian Pacific Journal Tropical Disease, 2012; 2 (3): 239–250.
27. Sezai Ercisli, Memnune Sengul, Hilal Yildiz, S. Sener, Boris Duralija, Sandra Voca. Phytoch- emical and antioxidant characteristics of medlar fruits (Mespilus germanica L.). Journal of Applied Botany and Food Quality, 2012; 85 (1): 86–90.
28. Yong-Jin Park, Ju Kyong Lee, Nam-Soo Kim. Simple sequence repeat polymorphisms (SS- RPs) for evaluation of molecular diversity and germplasm classification of minor crops. Mole- cules, 2009; 14(11): 4546–4569.

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